ABSTRACT

Objective:

Comparison of the effects of twin pregnancies using assisted reproductive technology (ART) and spontaneous twin pregnancies on maternal-fetal parameters.

Methods:

A total of 5,670 patients who were deliveried in our clinic between 01.01.2011 and 31.12.2017 were analyzed retrospectively. The fetomaternal results of 94 mothers who deliveried to twins and their infants were compared.

Results:

Of the 5,670 patients who gave birth, 94 were twins (1/60) and 8 were triplets (1/709). Twin births account for 92% of multiple births. The incidence of spontaneous twin pregnancy was 1/77 and constituted 79% of all the twin deliveries. The effect of ART on was found to be 21% [17% in vitro fertilization (IVF), 4% ovulation induction].

Mean maternal age was 29.25±6.00 years in twin pregnancy deliveries, 28.50±6.09 years in mothers who had spontaneous twin group, and 32.54±5.09 years in twin births due to ART.

When anemia was excluded, maternal morbidity was 27% in twin pregnancy deliveries. Pre-eclampsia was determined in 19% of the cases and anemia in 63%. Delivery before 37 gestational weeks was recorded in 72.3% of IVF twins and 70.2% of spontaneous twins. The rate of live births was found to be higher in spontaneously conceived twins (p=0.004). The fetal mortality rate was 5.4% in spontaneous twin pregnancies and 20% in twin pregnancies with ART (p=0.003). The perinatal mortality index was determined to be 81 in spontaneously conceived twins, and 250 in the twin pregnancies obtained with ART (p=0.006).

Conclusion:

Premature birth and fetal mortality rates are higher in twin pregnancies obtained with ART than in spontaneous twins. The mortality associated with multiple pregnancies may be reduced with preventive measures such as low-dose gonadotropin use, single embryo transfer, and multifetal embryo reduction.

INTRODUCTION

The pregnancy rate using assisted reproductive technology (ART) has increased significantly in the last four decades. Although ART usage rates in infertile patients are reported as 11% in the United States of America (USA) , this rate is estimated to be higher (1). Twin pregnancy rates due to ART have been reported as 18% in the USA (1). After ART applications had their place in reproductive biology clinical practice, the incidence of twin birth increased up to 1-4% (1-6). Although the increase in pregnancy rates was pleasing at first, the significant increase in multiple pregnancy rates due to success-oriented practices such as (i) advanced age, (ii) high dose drug use, (iii) multiple embryo transfer, both maternal mortality and maternal mortality. It leads to increased morbidity, as well as fetal morbidity and mortality (6,7). Parallel to the increase in the rates of gestational diabetes, gestational hypertension and preeclampsia in women who conceive after ART, a significant increase in the prevalence of preterm labor, low birth weight infants, respiratory distress syndrome and cerebral palsy draws attention (2,6-11). This study was carried out retrospectively in order to draw attention to the increase in maternal, fetal and perinatal mortality and morbidity rates in twin pregnancies due to ART. Spontaneous twin pregnancies were included in the control group, and maternal, fetal and perinatal morbidity and mortality differences were compared with twins due to ART.

GİRİŞ

Son dört dekatta yardımcı üreme teknikleri (YÜT) kullanılarak oluşan gebelik oranı anlamlı bir şekilde artmıştır. YÜT’nin infertil hastalarda kullanım oranları Amerika Birleşik Devletleri’nde (ABD) %11 olarak bildirilmekle birlikte bu oranın daha yüksek olduğu tahmin edilmektedir (1). ABD’de YÜT’e bağlı ikiz gebelik oranları %18 olarak rapor edilmiştir (1). YÜT uygulamalarının üreme biyolojisinde kullanıma girmesinden sonra ikiz doğum insidansı %1-4’lere kadar yükselmiştir (1-6). Ancak gebelik oranlarındaki artış ilk etapta sevindirici olsa da YÜT uygulanan hasta grubunun (i) ileri yaşta olması, (ii) yüksek doz ilaç kullanımı, (iii) birden fazla embriyo transferi gibi başarı odaklı uygulamalar nedeniyle çoğul gebelik oranlarının belirgin olarak artması hem maternal hem de fetal morbidite ve mortalitede artışa yol açmaktadır (6,7). YÜT sonrası gebe kalan kadınlarda gestasyonel diyabet, gebelik hipertansiyonu ve preeklampsi oranlarındaki artışa paralel olarak preterm doğum, düşük doğum ağırlıklı bebekler, respiratuvar distres sendromu ve serebral palsi görülme sıklığında da belirgin bir artış dikkati çekmektedir (2,6-11).  Bu çalışma YÜT’e bağlı gelişen ikiz gebeliklerde maternal, fetal ve perinatal mortalite ve morbidite oranlarında görülen artışa dikkat çekmek amacıyla retrospektif olarak yapıldı. Kontrol grubuna ise spontan ikiz gebelikler dahil edilerek YÜT’e bağlı ikizler ile aralarındaki maternal, fetal ve perinatal morbidite ve mortalite farkları karşılaştırıldı.

METHODS

This retrospective study was conducted after the approval of the Ethics Committee of Hatay Mustafa Kemal University (approval number: 06, approval date: 05/09/2019). The files of 5,670 pregnant women who admitted to our clinic between 01.01.2011 and 31.12.2017 and gave birth for 20 weeks or later, 500 grams (gr) or more were retrospectively analyzed. Patient consent was obtained in order to use the registered data of the patients. Multiple pregnancy records were made among the pregnant women who gave birth, and the clinical and demographic data of the patients were recorded. Maternal parameters subjected to registration process were; age, form of multiple pregnancy [spontaneous, intrauterine insemination, in vitro fertilization (IVF)], gravida, parity, abortion, number of surviving children, last menstrual period. The fetal parameters recorded were; week of birth, birth weight, mode of delivery, 1^st and 5^th minutes APGAR scores were determined. When it comes to maternal complications; physiological anemia of pregnancy, preeclampsia and gestational diabetes were taken into consideration. As a fetal complication, preterm birth, premature rupture of membranes, oligo-polyhydramnios, low birth weight baby, birth weight incompatibility, presence of fetal anomaly, fetal death were taken into consideration.

Multiple pregnancies with live births were classified as immature, premature and mature according to the following criteria; births between 20^th and 28^th weeks were recorded as early preterm (immature) births, births between 28^th and 37^th weeks as preterm birth (premature), and births at 37^th weeks and above as term (mature) births. In the classification made according to fetal birth weight; Babies born 2,500 gr and above were considered as normal birth weight, babies born below 2,500 gr low birth weight. In twin pregnancy deliveries, if there is a difference of 25% or more in terms of birth weight between twins, it was recorded as discordance. The perinatal mortality index (PMI) was calculated according to the formula below.

$\mathrm{PMI}=\left(\frac{(\mathrm{Number}\mathrm{Of}\mathrm{Stillborn}\mathrm{Babies}+\mathrm{Number}\mathrm{of}\mathrm{babies}\mathrm{dying}\mathrm{within}\mathrm{the}\mathrm{first}7\mathrm{days}\mathrm{after}\mathrm{birth})}{\mathrm{Number}\mathrm{of}\mathrm{all}\mathrm{babies}\mathrm{born}\left(\mathrm{alive}\mathrm{and}\mathrm{dead}\right)}\right)\mathrm{x}1000$

Statistical Analysis

SPSS 21 (SPSS Inc. Chicago, Illinois, USA) package program was used for statistical analysis of the obtained data. Spontaneous and ART twin births were compared with each other in terms of clinical, demographic, maternal and fetal complications. Student t-test was used for analysis of continuous variables. The obtained results were given as mean ± standard deviation or as n (%). Chi-square (χ²) tests were used for the analysis of non-continuous variables. P<0.05 was considered significant.

YÖNTEMLER

Bu retrospektif çalışma Hatay Mustafa Kemal Üniversitesi Etik Kurul onayı (onay numarası: 06, onay tarihi: 05/09/2019) alınmasını takiben gerçekleştirildi. Kliniğimize 01.01.2011-31.12.2017 tarihleri arasında başvuran, 20 hafta ve üzerinde, 500 gram (gr) veya üzerinde doğum yapan 5.670 gebenin dosyaları retrospektif olarak incelendi. Hastalara ait kayıtlı verilerin kullanılabilmesi için hasta onamı alındı.  Doğum yapan gebeler arasından çoğul gebelik kayıtları çıkartılarak hastaların klinik ve demografik verileri kaydedildi. Kayıt işlemine tabi tutulan maternal parametreler sırasıyla; hastanın yaşı, çoğul gebeliğin oluşum şekli [spontan, aşılama, tüp bebek (IVF)], gravida, parite, abortus, yaşayan çocuk sayısı, son adet tarihi olarak belirlendi. Kayıt yapılan fetal parametreler ise; doğum haftası, doğum ağırlığı, doğum şekli, 1. ve 5. dakika APGAR skorları olarak belirlendi. Maternal komplikasyon denildiğinde ise; gebeliğin fizyolojik anemisi, preeklampsi ve gestasyonel diyabet dikkate alındı. Fetal komplikasyon olarak ise; erken doğum, erken membran rüptürü, oligo-polihidramnios, düşük doğum ağırlıklı bebek, doğum ağırlığı uyumsuzluğu, fetal anomali varlığı, fetal ölüm dikkate alındı.

Canlı doğum gerçekleşen çoğul gebelikler aşağıdaki kriterlere göre immatür, prematür ve matür olarak sınıflandırıldı; 20-28. haftalar arası doğumlar erken preterm (immatür) doğum, 28-37. haftalar arası doğumlar preterm doğum (prematür), 37. hafta ve üzerindeki doğumlar ise term (matür) doğum olarak kaydedildi. Fetal doğum ağırlıklarına göre yapılan sınıflandırmada ise; 2.500 gr ve üzerinde doğan bebekler normal doğum ağırlıklı, 2.500 gr’den küçük doğan bebekler düşük doğum ağırlıklı olarak kabul edildi. İkiz gebelik doğumlarında ise ikizler arasında doğum ağırlıkları bakımından %25 ve üzeri fark var ise uyumsuzluk (diskordans) olarak kaydedildi. Perinatal mortalite indeksi (PMİ) aşağıdaki formüle göre hesaplandı.

$\mathrm{PMI}=\left(\frac{\mathrm{Ölü}\mathrm{Doğan}\mathrm{Bebek}\mathrm{Sayısı}+\mathrm{Doğumdan}\mathrm{Sonraki}\mathrm{ilk}7\mathrm{gün}\mathrm{içinde}\mathrm{Ölen}\mathrm{Bebek}\mathrm{Sayısı}}{\mathrm{Tüm}\mathrm{Doğan}\mathrm{Bebek}\mathrm{Sayısı}\left(\mathrm{Canlı}\mathrm{ve}\mathrm{Ölü}\right)}\right)\mathrm{x}1000$

İstatistiksel Analiz

Elde edilen verilerin istatistiksel analizleri için, SPSS 21 (SPSS Inc. Chicago, Illinois, USA) paket programı kullanıldı. Spontan ve YÜT ikiz doğumları klinik, demografik, maternal ve fetal komplikasyonlar açısından birbiri ile karşılaştırıldı. Sürekli değişkenlerin analizi için, Student t-test uygulandı. Elde edilen sonuçlar ortalama ± standart sapma şeklinde veya n (%) olarak verildi. Sürekli olmayan değişkenlerin analizi için ki-kare (χ²) testleri uygulandı. P<0,05 anlamlı olarak kabul edildi.

RESULTS

Out of 5,670 pregnant women 102 were deliveries due to multiple pregnancy. Of the 102 multiple births, 94 (92.2%) were twin and 8 (7.8%) were triplet births. Seventy-four of the twins (78.7%) were spontaneous twins, 20 of them (21.2%) were twin pregnancies due to ART. Sixteen of ART twins were obtained due to IVF and 4 of them were due to ovulation induction (OI). Among all pregnant women, the incidence of multiple pregnancy was 1/56, twin pregnancy incidence 1/60, and triplet pregnancy incidence 1/709. In our records, there were no quadruplet and quintet pregnancies that occurred either due to ART or spontaneously.

While the spontaneous twin birth incidence was 1/77, the incidence of twin births due to ART was found to be 1/283.5 (Table 1a). Average maternal age was found to be 29.25±6.00 years in general twin pregnancy deliveries, 28.50±6.09 years for mothers who had spontaneous twins, and 32.54±5.09 years for mothers with twins due to ART. The average age of mothers who gave birth to twins due to ART was found to be higher than mothers who gave birth to twins spontaneously (p<0.007) (Table 1a, Graphic 1). While 9.46% of spontaneous twins were over 35 years old, 35% of ART twins were over 35 years old and the difference was found to be significant (p<0.01) (Table 1a, Graphic 2).

Gravida (spontaneous 3.02±1.60; ART 1.64±0.84; p=0.004), parity (1.72±1.30; 0.43±0.76; p=0.001), mean living child (1.49±1.30; 0.36±0.74; p=0.003) in spontaneous twins was found to be significantly higher than the ART twins (Table 1a, Graphic 3). The number of babies born alive was found to be significantly higher in spontaneous twin births compared to twin births due to ART (95%, 80%, p=0.004, Table 1a, Graphic 4). No significant difference was found between spontaneous and ART twins in terms of abortion rates (0.33±0.76 vs 0.21±0.43; p=0.51). When anemia was excluded in all twin pregnancies, maternal morbidity was found as 27%. Anemia was detected in 63% of the cases who gave birth to twins (61% spontaneous twin, 70% due to ART), and 19% preeclampsia (20% in spontaneous twins; 15% in twins due to ART).

While the week of delivery was 35.18±3.27 weeks in spontaneous twins, it was found as 34.27±4.20 weeks in ART twins (Table 1b, Graphic 5). When twin births were evaluated in terms of APGAR scores, 1^st minute APGAR was found as 6.30±1.87 points in spontaneous twins and 6.21±2.02 points in ART twins. There was no difference between the groups in terms of 1^st minute APGAR values (p=0.383), while 5^th minute APGAR values were 8.12±1.56 points in spontaneous twins and 7.42±2.11 points in twins with ART (p=0.103, Table 1b, Graphic 5).

While the mean birth weight was 2223.87±603.97 g in spontaneous twin pregnancies, it was found similar as 2270.50±811.02 g in twin pregnancies due to ART (p=0.77). While it was 34% in spontaneous twins who were born 2,500 gr and above, it was found as 62.5% in ART twins (p=0.031; Table 1b). Low birth weight was found as 53.57% in spontaneous twin pregnancy deliveries and 18.75% in ART twins (p=0.056). Discordance between fetuses is shown in Table 1b as SGA, AGA, LGA. Cleft lip, cleft palate, left ventricular cyst in the heart, unilateral polycystic kidney and bilateral ventriculomegaly were found as fetal anomalies. Due to the low number of cases in terms of fetal anomalies, no comparison could be made between the groups.

While both fetuses were stillborn in two of the spontaneous twin pregnancies; In the remaining four deliveries, one of the fetuses was born alive and the other was dead. Both fetuses were stillborn in one of the twin pregnancy deliveries caused by ART. When the gender of stillborn twin babies were also evaluated, the rate of male babies was found to be higher in both spontaneous and ART-induced twins (Table 1b). When all twins were examined, 54% of the babies were female. The frequency of female babies was found to be higher in spontaneous twins compared to ART twins (61%; 30%, respectively; p<0.001; Table 1b).

Fetal mortality rate was 5.4% in spontaneous twin births and 20% in ART twins (p=0.003). Neonatal mortality rate was 5.4 in spontaneous twins; and 6.25 in twins connected to ART. PMI was 81 in spontaneous twins and 250 in ART twins (p<0.006), 117 in all twins.

DISCUSSION

In the USA, 11% of all newborns are reported to occur following the infertility treatment (1). 95% (1) of all multiple pregnancies in the USA, 99% (3) in the United Kingdom (UK), and 91% in Turkey Güler et al. (4) reported to have twin pregnancy births. Of the 102 multiple births in our clinic, 94 (92.2%) were twins and 8 (7.8%) were triplets. Our data are consistent with literature data. It has been reported that 18% of twin births in the USA, 18% in the UK, 23.3% in Australia and 41% in Italy were caused by ART (1,3,12,13). It was found that 74 (78.72%) of the twins born in our clinic were spontaneous and 20 (21.27%) developed with ART, our findings were consistent with the literature. Güler et al. (4) reported that 66.6% of the twins were with ART and 33.4% were spontaneous. We found that 16 (80%) of ART twin births were caused by IVF and 4 (20%) of them were caused by OI. Among all pregnant women, the incidence of multiple pregnancy was 102/5670 (1/56), twin pregnancy incidence 94/5670 (1/60), and triplet pregnancy incidence 8/5670 (1/709) (Table 1a). Twin births constitute 3-4% of all births in the USA (1), Bardis et al. (3) reported that 1.45% of them were twin pregnancy births in Australia. Güler et al. (4) stated the rate of twin birth as 2.7% in their study. Samani et al. (5) reported the rate of multiple births in Iran as 1.48%. Spontaneous twin birth rate has been reported as 0.6% to 2.79% (14), and the rate of twin births among those who underwent ART has been reported as 6.8% to 8.44% (3,5,15).

In studies, the average age of mothers who gave birth to twins with ART was found to be higher than the age of mothers who gave birth spontaneously (3,4,12). In Australia, Wang et al. (12) reported that 25.8% of spontaneous twins are 35 years old and over, and 45.2% of ART twins are over 35 years old. In our study, 9.46% of spontaneous twins were over 35 years old, while 35% of ART twins were over 35 years old (p=0.010, Table 1a, Graphic 2).

In the gravida, parity, living child examination, it is reported in the literature that ART is predominant in mothers who gave birth to primipara twins, and spontaneous twins are dominant in multipara. It has been reported that the number of surviving children of mothers who gave birth to twins spontaneously is higher (7,10,12,13). Gravida, parity, and the mean of living children were found to be higher in spontaneous twins compared to ART twins (Table 1a, Graphic 3, 4). In our study, we obtained findings compatible with the literature.

The rate of preterm birth (<37 weeks) in twin pregnancy deliveries ranges between 36.58% and 79% (2,8,9). It has been reported that preterm delivery in ART pregnancies is higher than spontaneous twin pregnancy deliveries due to ART procedure and the underlying reasons (6,7,12,13,16,17). In our study, the week of delivery was 35.18±3.27 weeks in spontaneous twins; 34.27±4.20 weeks in ART twins, similar to the literature. It has been reported that the live birth rate is lower in ART twin pregnancy births than in spontaneous twin pregnancy births (11-13). In our study, the number and rate of babies born alive were found to be higher in spontaneous twins (95%; 80%, respectively; p=0.004, Table 1a, Graphic 4).

In the literature, it has been reported that maternal morbidity and complications are higher in ART pregnancies than in spontaneous pregnancies (11-13). Preeclampsia rate was reported as 19.6% in ART pregnancies and 13.6% in spontaneous pregnancies (8,11,13). In twin pregnancy deliveries, when anemia was excluded, we found maternal morbidity 27%, anemia 63% and preeclampsia 19%. No significant difference could be found between maternal complications in spontaneous twin pregnancy delivery and ART twin pregnancy deliveries, this may be due to the low number of our cases.

There are publications reporting that baby birth weight is significantly higher than spontaneous twin pregnancy births in ART-induced twin pregnancy deliveries (3,11) and there are also publications reporting that there is no difference (4,6,12,13). There was no significant difference between the groups in terms of birth weight. In spontaneous twins, births of 2,500 gr and above were found to be 34%; It was 62.5% in ART twins (p=0.031; Table 1b). The reason may be that mothers who were pregnant with ART had better perinatal follow-up than mothers who were spontaneously pregnant. There was no significant difference between the groups in terms of the presence of discordance between fetuses. Comparison could not be made in terms of fetal anomalies due to the low number of cases.

There are publications (13) reporting that APGAR 1^st-5^th minute scores are higher (4) or no difference (12) in spontaneous twin pregnancy deliveries. We also did not find any difference between 1^st-5^th minutes APGAR values  (Table 1b).

There was no significant difference between the groups in terms of the sex of twin babies born alive. When the gender of stillborn twin babies was evaluated, male baby gender was found to be 81.25% (Table 1b). This is because in fetal life, female sex fetuses are more prone to survival than male sex fetuses. Özkan et al. (18) reported male gender as 54% in his study on fetal autopsies. When all twins were examined, 57.5% of the babies were female babies. Yumru et al. (19) reported the male baby gender at birth as 52%. In our study, the frequency of female babies was found to be higher in spontaneous twins compared to ART twins (spontaneous 60.7%; ART 44%).

While neonatal death was reported as 2.1% in ART pregnancies in twins; this rate is 1.8% in spontaneous pregnancies (12). In our study, neonatal mortality was 5.4% in spontaneous twins; 6.25% of twins born with ART; fetal mortality was 5.4% in spontaneous twin pregnancy and 20% in ART twins (p=0.003). In singleton, twin and multiple pregnancy deliveries resulting from ART; it has been reported that perinatal mortality and morbidity are higher than spontaneous pregnancy deliveries (3,6,12,13). We found PMI as 117 in all twins, 81 in spontaneous twins, and 250 in ART twins. Our findings are consistent with the literature data.

Study Limitations

The limitations of our study are the retrospective nature of our study and the low number of our cases as 94. We hope that the studies on a much larger number of cases will contribute significantly to this issue.

TARTIŞMA

ABD’de tüm yeni doğanların %11’nin infertilite tedavisi sonrası gerçekleştiği bildirilmektedir (1). Tüm çoğul doğumların ABD’de %95’ini (1) , İngiltere’de %99’unu (3) , Güler ve ark. (4) ise %91’ini ikiz gebelik doğumlarının oluşturduğu bildirilmiştir. Kliniğimizdeki 102 çoğul doğumun 94’ü (%92,2) ikiz, 8’i (%7,8) üçüz doğumdu. Bizim verilerimiz literatür verileri ile uyum göstermektedir. ABD’de ikiz doğumların %18’inin,  İngilterede %18’inin, Avustralya %23,3’nün ve İtalya’da %41’nin YÜT sonucu oluştuğu bildirilmiştir (1,3,12,13). Kliniğimizde doğan ikizlerin 74’ünün (%78,72) spontan, 20’sinin (%21.27) YÜT ile geliştiği tespit edildi, bulgularımız literatür ile uyumludur. Güler ve ark. (4) ikizlerin %66,6’sının YÜT ile, %33.4’ünün ise spontan olarak oluştuğunu bildirmiştir.  YÜT ikiz doğumlarının 16 (%80)’sının IVF, 4’ünün (%20) Oİ sonucu oluştuğunu bulduk. Bütün gebeler arasında, çoğul gebelik insidansı 102/5.670 (1/56), ikiz gebelik insidansı 94/5.670 (1/60), üçüz gebelik insidansı 8/5.670 (1/709) olarak tespit edildi (Tablo 1a). Tüm doğumların ABD’de %3-4’ünü ikiz doğumlar oluşturduğu (1), Bardis ve ark. (3) Avustralya’da %1.45’ini ikiz gebelik doğumları oluşturduğunu bildirmiştir. Güler ve ark. (4) yapmış oldukları çalışmada ikiz doğum oranını %2,7 olarak belirtmiştir. Samani ve ark. (5) İran’da çoğul doğum oranını %1,48 olarak bildirmiştir.   Spontan ikiz doğum oranı ise %0,6 ila %2,79 (14), YÜT uygulananlar arasında ikiz doğum oranı ise %6,8 ile %8,44 olarak bildirilmiştir (3,5,15).     

Yapılan çalışmalarda YÜT ile ikiz doğum yapan annelerin yaş ortalaması, spontan gebe kalıp doğum yapan annelerin yaşlarından daha yüksek bulunmuştur (3,4,12). Avusturalya’da Wang ve ark. (12) spontan ikizlerin %25,8’inin 35 yaş ve üzerinde, YÜT ikizlerinin ise %45,2’sinin 35 yaş üzerinde olduğunu bildirmiştir. Bizim çalışmamızda ise spontan ikizlerin %9,46’sı 35 yaş üzerindeyken, YÜT ikizlerinin %35’i 35 yaş üzerindeydi (p=0,010, Tablo 1a, Grafik 2).

Literatürde gravida, parite, yaşayan çocuk incelemesinde, primipar ikiz doğuran annelerde YÜT’nin, multiparlarda spontan ikizlerin baskın olduğu bildirilmektedir. Spontan ikiz annelerin yaşayan çocuk sayısı ortalamasının daha yüksek olduğu bildirilmektedir (7,10,12,13). Gravida, parite, yaşayan çocuk ortalaması spontan ikizlerde YÜT ikizlerine göre daha yüksek bulundu (Tablo 1a, Grafik 3, Grafik 4). Bizim araştırmamızda da literatürle uyumlu bulgular elde ettik.

İkiz gebelik doğumlarında preterm doğum (<37 hafta) oranı %36,58-%79 arasında değişmektedir (2,8,9). Gerek YÜT işlemine bağlı, gerekse altta yatan sebeplere bağlı olarak YÜT gebeliklerinde preterm doğumun spontan ikiz gebelik doğumlarından daha yüksek olduğu bildirilmiştir (6,7,12,13,16,17). Bizim çalışmamızda doğum haftası, spontan ikizlerde 35,18±3,27 hafta; YÜT ikizlerinde 34,27±4,20 hafta olarak literatürle benzer bulundu. Canlı doğum oranının YÜT ikiz gebelik doğumlarında, spontan ikiz gebelik doğumlarından daha düşük olduğu rapor edilmiştir (11-13). Bizim çalışmamızda da canlı doğan bebek sayı ve oranı spontan ikizlerde daha yüksek bulundu (sırasıyla, %95; %80; p=0,004, Tablo 1a, Grafik 4).

Literatürde YÜT gebeliklerinde maternal morbidite ve komplikasyonların spontan gebeliklerden daha yüksek olduğu bildirilmiştir (11-13). Preeklampsi oranı YÜT gebeliklerinde %19,6, spontan gebeliklerde 13,6 olarak bildirilmiştir (8,11,13). İkiz gebelik doğumlarında, anemi dışlandığında, maternal morbideteyi %27, anemiyi %63 ve preeklampsiyi %19 olarak bulduk. Spontan ikiz gebelik doğumu ve YÜT ikiz gebelik doğumlarında maternal komplikasyonlar arasında anlamlı bir fark tespit edilemedi, bunun sebebi olgu sayımızın azlığından kaynaklanıyor olabilir.

YÜT’e bağlı ikiz gebelik doğumlarında bebek doğum ağırlığının spontan ikiz gebelik doğumlarından anlamlı derecede fazla olduğunu bildiren yayınların yanı sıra (3,11) fark olmadığını bildiren yayınlar da mevcuttur (4,6,12,13). Doğum ağırlığı bakımından gruplar arasında anlamlı fark tespit edilemedi. 2.500 gr ve üzerindeki doğumlar spontan ikizlerde %34; YÜT ikizlerinde %62,5 olarak tespit edildi (p=0,031; Tablo 1b). Bunun sebebi, YÜT ile gebe kalan annelerin spontan gebe kalan annelerden daha iyi perinatal takip almış olabilir. Fetüsler arasında diskordansın varlığı açısından gruplar arasında anlamlı fark bulunamadı. Fetal anomaliler bakımından olgu sayısının azlığından dolayı kıyaslama yapılamadı.

APGAR 1-5 dakika skorları spontan ikiz gebelik doğumlarında daha yüksek olduğunu (4) veya fark olmadığını (12) bildiren (13) yayınlar mevcuttur. Biz de 1-5 dakika APGAR değerleri arasında fark bulamadık (Tablo 1b).

Canlı doğan ikiz bebeklerin cinsiyetleri açısından gruplar arasında anlamlı fark saptanmadı. Ölü doğan ikiz bebeklerin cinsiyetleri değerlendirildiğinde, erkek bebek cinsiyeti %81,25 bulundu (Tablo 1b). Bunun sebebi fetal hayatta, kız cinsiyetli fetüsler, erkek cinsiyetli fetüslerden yaşama daha dirençlidir. Özkan ve ark. (18) fetal otopsilerde yapmış olduğu çalışmada, erkek cinsiyetini %54 olarak bildirmiştir. Tüm ikizler incelendiğinde bebeklerin %57,5’ini kız bebekler oluşturmaktaydı. Yumru ve ark. (19) yapmış olduğu çalışmada doğumda erkek bebek cinsiyetini %52 olarak bildirmiştir. Bizim çalışmamızda spontan ikizlerde YÜT ikizlerine göre kız bebek sıklığı daha fazla tespit edildi (spontan %60,7; YÜT %44).

İkizlerde neonatal ölüm YÜT gebeliklerinde %2,1; spontan gebeliklerde %1,8 olarak bildirilmiştir (12). Bizim çalışmamızda, neonatal mortalite spontan ikizlerde %5,4; YÜT’e bağlı ikizlerde ise %6,25; fetal mortalite spontan ikiz gebelik doğumlarında %5,4, YÜT ikizlerinde ise %20 (p=0,003) bulundu. YÜT sonucu gelişen tekil, ikiz ve çoğul gebelik doğumlarında, perinatal mortalite, morbiditenin, spontan gebelik doğumlarından daha yüksek olduğu rapor edilmiştir (3,6,12,13). Tüm ikizlerde PMİ 117, spontan ikizlerde 81, YÜT ikizlerinde ise 250 olarak saptadık. Bulgularımız literatür verileriyle uyumludur.

Çalışmanın Kısıtlılıkları

Çalışmamızın retrospektif olması, olgu sayımızın 94 olarak az sayıda olması çalışmamızın kısıtlılık yönleridir. Çok daha fazla sayıda olgu üzerinde yapılacak olan çalışmaların bu konuya önemli ölçüde katkı sağlayacağını ümit ediyoruz.

CONCLUSION

We can list the measures that can be taken to reduce the maternal and fetal negative effects of ART-related multiple pregnancies as follows: Single embryo transfer should be done in IVF. In cases where 3 or more follicles develop during OI and intrauterin insemination, IVF application or cycle cancellation should be performed. In IVF cycles, ovarian stimulation should be done with low dose gonadotropin. Embryo cryopreservation is required for cycles with hyperstimulation in IVF. While the patient is preparing for IVF, estradiol level should be kept below 3,000 pg/mL as much as possible. We can reduce the occurrence of multiple pregnancies due to ART by using analogues instead of human chorionic gonadotropin and embryo cryopreservation in triggering ovulation in cases where estradiol level is 3,000 pg/mL ≤.