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The Effects of <i>Prunus Laurocerasus</i> Fruit Extract on Oxidative and Endoplasmic Reticulum Stress Responses in Doxorubicin-induced Cardiac Damage
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Original Investigation
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The Effects of Prunus Laurocerasus Fruit Extract on Oxidative and Endoplasmic Reticulum Stress Responses in Doxorubicin-induced Cardiac Damage

Selma Cırrık 1
Emel Kabartan 2
Gülay Hacıoğlu 3
Emine Gülçeri Güleç Peker 4
1. Ordu University Faculty of Medicine, Department of Physiology, Ordu, Turkey
2. Ordu University Ulubey Vocational School, Laboratory and Veterinary Health Program, Ordu, Turkey
3. Giresun University Faculty of Medicine, Department of Physiology, Giresun, Turkey
4. Giresun University Faculty of Engineering, Department of Basic Sciences, Giresun, Turkey
No information available.
No information available
Received Date: 18.11.2023
Accepted Date: 06.04.2024
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ABSTRACT

Objective:

In this study, the effects of Prunus laurocerasus fruit extract (PLE), which is known for its high levels of phenolic compounds and antioxidant efficiency, on doxorubicin (DXR)-induced cardiotoxicity were examined.

Methods:

Male Sprague-Dawley rats were randomly assigned to the Control, DXR, PLE500+DXR, and PLE1000+DXR groups (n=6). PLE was orally administered to the animals in the PLE500+DXR (500 mg/kg) and PLE1000+DXR (1000 mg/kg) groups for 2 weeks. DXR was injected (15 mg/kg, intraperitoneally) 2 days before sacrification in the DXR, PLE500+DXR, and PLE1000+DXR groups. At the end of the study period, serum troponin I levels, myocardial superoxide dismutase (SOD) and catalase (CAT) activities, malondialdehyde (MDA), glutathione (GSH), glucose-regulated protein (GRP)78, and pro-caspase 12 levels were measured.

Results:

In the DXR group, serum troponin I concentration significantly increased (p<0.001), however PLE treatment significantly reduced this DXR-induced increment (p<0.001 and p<0.05 in the PLE500+DXR and PLE1000+DXR groups, respectively). DXR-induced increase in myocardial MDA (p<0.001) significantly reduced in the PLE500+DXR (p<0.001) and PLE1000+DXR (p<0.001) groups. However, DXR-induced changes, such as GSH depletion (p<0.001), reduced CAT (p<0.001) and SOD (p<0.001) activities, increased GRP78 (p<0.01) and decreased pro-caspase 12 (p<0.001) levels, were not significantly affected by PLE treatment.

Conclusion:

The present results indicate that pretreatment with PLE reduced, but not completely prevented, cardiac damage induced by DXR. While a reduction in oxidative stress appears to play a role in the partial protective effect of PLE treatment, endoplasmic reticulum stress appears to have no role. Further research is necessary to understand the mechanisms of action underlying PLE treatment.