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The Protective Effects of Omega-3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Liver Apoptosis
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Original Investigation
VOLUME: 1 ISSUE: 1
P: 9-11
September 2011

The Protective Effects of Omega-3 Fatty Acids and Sesame Oil on Cyclosporine-A Induced Liver Apoptosis

J Acad Res Med 2011;1(1):9-11
DOI: 10.5152/jarem.2011.03
Azize Yasemin Göksu Erol 1
Aziz Bülbül 2
Gülcan Avcı 3
Mehmet Özdemir 4
Özlem Akkaya 5
1. Department of Histology and Embryology, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
2. Department of Physiology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
3. Department of Biochemistry, Faculty of Veterinary Medicine, Afyonkocatepe University, Afyonkarahisar, Turkey
4. Department of Pharmacology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
5. Department of Histology and Embryology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey
No information available.
No information available
Received Date: 06.08.2011
Accepted Date: 12.09.2011
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ABSTRACT

Objective:

Cyclosporine A (CsA) is the most effective and widely used immunosuppressant in solid organ transplantation and autoimmune diseases. Despite its benefits, the clinical use of CsA is limited by its nephrotoxic and hepatotoxic properties. Omega-3 fatty acids (O-3) comprise a family of unsaturated fatty acids that consist of α-linolenic acid, eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) which has antioxidant properties. On the other hand, sesame oil (SO) contains many constituents, including a variety of antioxidant vitamins, glycerol esters of different fatty acids, and sesamol, which was recently reported to be a potent antioxidant, etc. In our study, we aimed to investigate the potential protective effects of O-3 and SO against CsA-induced liver apoptosis in rats.

Methods:

Seven groups of 7 rats/group were treated with saline/drugs for 15 days. In group 1, only saline was given orally (Po). In group 2, subcutaneous (s.c.) CsA was administered (15 mg/kg/day). In group 3, CsA (15 mg/kg/day; sc) and SO (1 ml/kg, Po) were given. In group 4, CsA (15 mg/kg/day; sc) and O-3 (100 mg/kg; Po) were given orally. In group 5, CsA (15 mg/kg/day; sc) and SO (1 ml/kg; Po) and O-3 (100 mg/kg; Po) were given synchronously. In group 6, only SO (1 ml/kg; Po) and in group 7 only O-3 (100 mg/kg, Po) were given. 24 h after the last treatments animals were sacrificed and livers were extracted. Apoptotic cells in liver tissues were counted using the TUNEL method.

Results:

In the group treated with CsA (Group 2), the number of apoptotic hepatocytes was significantly higher than the control group (Group 1) (p<0.001). Groups treated with SO (Group 3) or O-3 (Group 4) or both (Group 5) in addition to CsA have a reduced apoptotic cell count compared to CsA-treated group, but still significantly higher than the control group (p<0.001 for all 3 groups). Furthermore, the apoptotic cell count in the groups treated with SO-alone and O-3-alone were as low as in the control group.

Conclusion:

We indicated for the first time that O-3 and SO treatment has a protective effect against apoptosis induced by CsA in rats. Moreover, our study also revealed that SO and O-3 do not show any pro-apoptotic effects on the liver. The mechanisms underlying hepatoprotection of SO and O-3 may be related to both their free radical scavenging properties and indirect effects as a regulator of antioxidative systems. Thus, SO and O-3 can be candidates as good chemoprotectants. (JAREM 2011; 1: 8-11)

Keywords: Cyclosporine A, sesame oil, omega-3 fatty acids, liver, apoptosis

References

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